
Optimizing Peptide Permeability Assays
Our work, presented at GRC 2025, focuses on overcoming the analytical and biological hurdles associated with peptide permeability testing. The MDCK-MDR1 cell model is a well-established system for evaluating P-glycoprotein (P-gp)-mediated transport, commonly used for small molecules. But peptides, due to their high molecular weight, lipophilicity, and strong intracellular binding, require a more nuanced approach.