Bioanalytical LC-MS/MS Assay List 

With 20+ years of working to GLP and GCP, we have developed an extensive number of LC-MS/MS bioanalytical methods in a wide range of species and matrices. These have been established/validated in line with the latest EMA/FDA guidance and applied in the quantitation of therapeutic drugs, metabolites and biomarkers.

Assay Experience

We have developed and validated numerous assays for non-proprietary molecules across a broad range of species and matrices. A selection of our extensive bioanalytical LC-MS/MS method experience is presented in the following list, but it’s not exhaustive.

If your analyte of interest isn’t listed, please get in touch and we can discuss setting up assays to support your project requirements.

 

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 Analyte name Calibration curve range
 (LLOQ-ULOOQ)		  Species Matrix  Anticoagulant Sample 
 volume (μL)		  Storage  temperature
Abiraterone 0.1 - 400 ng/mL Human Plasma K2  EDTA 50 -80 or -20
Albuterol 4 - 2000 pg/mL Human Plasma K2  EDTA 200 -80 or -20
L-Albuterol 4 - 2000 pg/mL Human Plasma K2  EDTA 200 -80 or -20
Aldosterone 5 - 500 pg/mL Human Plasma K2  EDTA 300 -80
Aldosterone 0.1 - 50 ng/mL Human Urine N/A 100 -80
Amoxicillin 50 - 20000 ng/mL Human Plasma K2  EDTA 100 -80 or -20
Amphotericin B (Encapsulated) 100 - 50000 ng/mL Dog Plasma K2  EDTA 100 -80 or -20
Amphotericin B (Free) 10 - 3000 ng/mL Dog Plasma K2  EDTA 100 -80 or -20
Amphotericin B (Encapsulated) 100 - 50000 ng/mL Human Plasma K2  EDTA 100 -80 or -20
Amphotericin B (Free) 10 - 3000 ng/mL Human Plasma K2  EDTA 100 -80 or -20
Amtriptyline & Nortriptyline 0.1/0.05 - 50/25
ng/mL		 Human Plasma K2  EDTA 50 -80 or -20
Androstenedione 0.1 - 100 ng/mL Human Plasma K2  EDTA 200 -20
Angiotensin I (Plasma Renin Activity) 0.2 - 200 ng/mL Human Plasma K2  EDTA 100 To be
confirmed
Apremilast 5 - 1000 pg/mL Human Plasma N/A 50 N/A
Aripiprazole Olanzapine Quetiapine Risperidone Paliperidone 5 - 500 ng/mL
0.2 - 100 ng/mL
5 -500 ng/mL
0.1 - 100 ng/mL
0.2 - 100 ng/mL		 Human Plasma K2  EDTA 100 -20
Atorvastatin
2-Hydroxy Atorvastatin 4-Hydroxy Atorvastatin		 20 - 20000 pg/mL
20 - 20000 pg/mL
10 - 1000 pg/mL		 Human Plasma K2  EDTA 100 -20
Baloxavir 1 - 80 ng/mL Human Plasma K2  EDTA 500 N/A
Beraprost sodium 5 - 500 pg/mL Human Plasma K2  EDTA 400 -80 or -20
Bile Acids (19 analytes) 1 - 500 ng/mL Human Plasma K2  EDTA 50 -80 or -20
Bile Acids (19 analytes) 1 - 500 ng/mL Human Urine K2  EDTA 50 -80 or -20
Budesonide 10 - 10000 pg/mL Human Plasma NaF / KOx 250 -20
Budesonide 5 - 1000 pg/mL Human Plasma K2  EDTA 25 -80 or -20
Budesonide/Formoterol 3/1 - 300/100 pg/mL Human Plasma K2  EDTA 50 N/A
Buprenorphine Norbuprenorphine 2 -1000 pg/mL
5 - 1000 pg/mL		 Human Plasma K2  EDTA 400 -20
C4
(7 -hydroxy-4-cholensten-3-one)		 2 -500 ng/mL Human Plasma Lithium Heparin 100 -20
Calcitriol 20 - 400 pg/mL Human Plasma K2  EDTA 50 -80 or -20
Candesartan/amlidipine 1/0.05 - 200/10
ng/mL		 Human Plasma K2  EDTA 200 -80 or -20
Cannabidiol 1 - 1000 ng/mL Human Plasma K2  EDTA 25 -80 or -20
Carbamazepine 5 - 1000 ng/mL Human Plasma K2  EDTA 100 -80 or -20
Carbamazepine 50 - 50000 ng/mL Human Plasma K2  EDTA 100 -80 or -20
Cinacalcet 0.1 - 50 ng/mL Human Plasma K2  EDTA 100 N/A
Ciprofloxacin 100 - 4500 ng/mL Human Plasma Lithium Heparin 50 -20
Cisplatin 10 - 500 ng/mL Human Plasma K2  EDTA 50 N/A
Clopidogrel Clopidogrel acid 10 - 5000 pg/mL
30 - 4000 ng/mL		 Human Plasma Lithium Heparin 100 -80
Clozapine 5 -1000 ng/mL Human Plasma K2  EDTA 50 -20
Colchicine 10 - 10000 pg/mL Human Plasma K2  EDTA 150 -80 or -20
Cortisol 1 - 500 ng/mL Human Plasma K2  EDTA 200 -20
Cortisol 0.2 - 20 ng/mL Human Saliva N/A 200 -20
Cortisol 0.6 - 300 ng/mL Human Urine N/A 200 -20
Cortisol
11-Deoxycortisol Corticosterone
11-Deoxycorticosterone		 1 - 250 ng/mL
20 - 2000 pg/mL
50 - 25000 pg/mL
20 - 1000 pg/mL		 Human Plasma K2  EDTA 200 -80
Cyclosporin A Tacrolimus Sirolimus 1 - 500 ng/mL
0.1 - 50 ng/mL
0.1 - 50 ng/mL		 Human Whole Blood K2  EDTA 50 -80
Cytarabine (encapsulated) 100 - 10000 ng/mL Human Plasma N/A 400 -80 or -20
Cytarabine (free) 10 - 2000 ng/mL Human Plasma K2  EDTA 50 -80 or -20
Daunorubicin/Cytarabine (Total) 44.8/100 -
4480/10000 ng/mL		 Human Plasma K2  EDTA 25 N/A
Daunorubicin (encapsulated) 44.8 - 44800 ng/mL Human Plasma K2  EDTA 400 N/A
Daunorubicin (free) 0.5 - 500 ng/mL Human Plasma K2  EDTA 50 -80 or -20
Desmosine 0.25 - 100 nM
(~0.13 - ~53 ng/mL)		 Human Urine N/A 300 -20
Desmosine Isodesmosine 30 - 2000 pM
(~16 - ~1050 pg/mL)		 Human Serum N/A 500 -20
Dexmedetomidine 20 - 5000 ng/mL Human Plasma K2  EDTA 50 -80 or -20
Diazepam 0.5 - 500 ng/mL
2 - 1000 ng/mL		 Human Plasma K2  EDTA 50
500		 -20
-80 or -20
Desmethyldiazepam 0.1 - 100 ng/mL Human Plasma K2  EDTA 50 -20
Diclofenac 20 - 2000 ng/mL Human Plasma K2  EDTA 600 -80 or -20
Digoxin 10 - 5000 pg/mL Human Plasma Lithium Heparin 100 -20
Doxorubicin (total) 50 - 50000 ng/mL Dog Plasma K2  EDTA 25 -80 or -20
Doxorubicin (encapsulated) 50 - 50000 ng/mL Dog Plasma K2  EDTA 25 -80 or -20
Doxorubicin (free) 1 - 400 ng/mL Dog Plasma K2  EDTA 150 -80 or -20
Doxorubicin (total) 50 - 50000 ng/mL Human Plasma K2  EDTA 25 -80 or -20
Doxorubicin (encapsulated) 50 - 50000 ng/mL Human Plasma K2  EDTA 100 -80 or -20
Doxorubicon (free) 1 - 400 ng/mL Human Plasma K2  EDTA 150 -80 or -20
Duloxetine 1 - 500 ng/mL
0.1 - 100 ng/mL		 Human Plasma Lithium Heparin 100
50		 -20
N/A
DSPC 500 - 50000 ng/mL Rat Plasma K2  EDTA 25 N/A
Eldcalcitol 10 - 300 pg/mL Human Serum N/A 40 -80 or -20
Epinastine 0.1 - 40 ng/mL Human Plasma K2  EDTA 50 -80 or -20
EPA & DHA (Total) 1 - 1000 ug/mL Human Plasma K2  EDTA 25 N/A
EPA & DHA (Free) 0.1 - 100 ug/mL Human Plasma K2  EDTA 400 N/A
Enoxacin 5 - 5000 ng/mL Human Plasma K2  EDTA 50 -20
Entacapone 2 - 2000 ng/mL Human Plasma K2  EDTA 50 -80 or -20
Estradiol 5 - 1000 pg/mL Human Serum N/A 500 -20
Ethambutol 100 - 40000 ng/mL Human Plasma K2  EDTA 50 N/A
Ethinylestradiol Levonorgestrel 10 - 2000 pg/mL
0.1 - 20 ng/mL		 Human Plasma Sodium Heparin 300 -20
Febuxostat 5 - 2500 ng/mL Human Plasma K2  EDTA 50 -80,20
Fluconazole 50 - 4000 ng/mL Human Plasma Lithium Heparin 50 -20
Fluconazole 50 - 50000 ng/mL Human Plasma K2  EDTA 20 -20
Fluticasone Propionate 1 - 500 pg/mL
0.5 - 200pg/mL		 Human Plasma NaF/KOx 500 -20
Fluticasone Propionate (High Range) Salmeterol 5 - 1000 pg/mL
1 - 250 pg/mL		 Human Plasma NaF/KOx 500 -20
Fluticasone Propionate (Low Range) Salmeterol 1 - 250 pg/mL Human Plasma NaF/KOx 500 -20
Fluticasone forate glycopoyoride Indacaterol 2 - 500 ng/mL
2 - 500 ng/mL
4 - 1000 ng/mL		 Human Plasma K2  EDTA 600 -80 or -20
Fluoxetine 0.1 - 25 ng/mL Human Plasma K2  EDTA 50 -80 or -20
Fluvastatin 1 - 1000 ng/mL Human Plasma K2  EDTA 50 -20
Fluvoxamine 5 - 1200 ng/mL Human Plasma K2  EDTA 50 -20
Formoterol 0.250 - 125 pg/mL Human Plasma NaF/KOx 500 -20
Furosemide 10 - 5000 ng/mL Human Plasma Lithium Heparin 100 -20
Gabapentin 50 - 25000 ng/mL Human Plasma K2  EDTA 25 -80 or -20
Gemfibozil Gemfibrozil 1-O-β-glucuronide 50 - 25000 ng/mL Human Plasma K2  EDTA 20 -20
Gemfibrozil & metabolite 0.1 - 50ng/mL Human Plasma K2  EDTA 25 -80 or -20
Glucagon 15 - 250 pg/mL Human Plasma K3  EDTA 200 -80
Glycopyorrate 2 - 800 pg/mL Human Plasma K2  EDTA 500 N/A
Guanfucin 1 - 500 ng/mL Human Plasma K2  EDTA 50 N/A
D-glucose 20 - 2000ug/mL Human Plasma K2  EDTA 50 -80 or -20
Hydromorphone Naloxone
Hydromorphone-3β-Glucuronide Naloxone-3β-Glucuronide		 10 - 2500 pg/mL
100 - 2500 pg/mL
100 - 25000 pg/mL
Low range: 100 - 25000 pg/mL
High range: 1000 - 250000 pg/mL		 Human Plasma K2  EDTA 200 -20
4β-Hydroxycholesterol 1 - 500 ng/mL Human Plasma K2  EDTA 50 -80
Ibuprofen 1 - 100 µg/mL Human Plasma Lithium Heparin 50 -20
Ibuprofen 0.2 - 100ng/mL Human Plasma K2  EDTA 50 -80 or -20
Ibuprofen (R and S) 0.1 - 50 µg/mL Human Plasma Lithium Heparin 50 -80
Indacaterol glycopyoride 4 - 1000 pg/mL
2 - 500 pg/mL		 Human Plasma K2  EDTA 500 -80 or -20
Indacaterol 2 - 500 pg/mL Human Plasma K2  EDTA 500 N/A
Insulin-Aspart 0.1 - 50 ng/mL Human Plasma K2  EDTA 250 -80 or -20
Ipratropium 4 - 2000 pg/mL Human Plasma K2  EDTA 400 -80 or -20
Irinotecan (Free) 2 - 1000 ng/mL Human Plasma K2  EDTA 100 -80 or -20
Irinotecan (encapsulated) 100 - 75000 ng/mL Human Plasma K2  EDTA 78 -80 or -20
Irinotecan (total) 100 - 75000 ng/mL Human Plasma K2  EDTA 25 -80 or -20
Irinotecan (Free) 2 - 1000 ng/mL Dog Plasma K2  EDTA 100 -80 or -20
Irinotecan (encapsulated) 100 - 75000 ng/mL Dog Plasma K2  EDTA 75 -80 or -20
Irinotecan (total) 100 - 75000 ng/mL Dog Plasma K2  EDTA 25 -80 or -20
Isoniazid 50 - 10000 ng/mL Human Plasma K2  EDTA 50 N/A
Itraconazole Hydroxyitraconazole 5 - 2000 ng/mL Human Plasma K2  EDTA 50 -20
Itraconazole & OH-Itraconazole 1 - 1000 ng/mL Human Plasma K2  EDTA 200 -80 or -20
Lacosamide 100 - 20000 ng/mL Human Plasma K2  EDTA 25 -80 or -20
Lamotrigine 50-25000ng/mL Human Plasma K2  EDTA 25 -80 or -20
Levetiracetam 100-40000ng/mL Human Plasma K2  EDTA 25 -80 or -20
Levodopa Carbidopa 3-OMD 50 - 7500 ng/mL
10 - 1500 ng/mL
200 - 30000 ng/mL		 Human Plasma K2  EDTA 50 -80
Levofloxacin 20 - 20000 ng/mL Human Plasma Sodium Heparin 50 -80
Levofolinic acid Methotrexate Levomefolic Acid 10 - 10000 ng/mL
5 - 5000 ng/mL
10- 10000 ng/mL		 Human Plasma K2  EDTA 200 -80 or -20
Linagliptin 0.1 - 10 ng/mL Human Plasma K2  EDTA 50 -80 or -20
Linezolid 50 - 20000 ng/mL Human Plasma K2  EDTA 25 -80 or -20
Lorazepam 0.5 - 50 ng/mL Human Plasma K2  EDTA 50 -80 or -20
LTE4 (Leukotriene E4) 8 - 500 pg/mL Human Urine N/A 1000 -80
LTE4 10 - 1000 ng/mL Human Urine K2  EDTA 50 -80 or -20
Menaquinone-7 0.5 - 400ng/mL Human Plasma K2  EDTA 400 -80 or -20
Metformin 2 - 1000ng/mL Human Plasma K2  EDTA 50 -80 or -20
Metoprolol 1 - 500ng/mL Human Plasma K2  EDTA 50 N/A
Metronidazole 50 - 1000ng/mL Human Plasma K2  EDTA 50 N/A
Mesalazine 2 - 1000ng/mL Human Plasma K2  EDTA 200 -80 or -20
Midazolam
1-OH Midazolam 4-OH Midazolam		 0.1 - 100 ng/mL Human Plasma K2  EDTA 100 -20
MMAE 40 - 10000 pg/mL Human Plasma K2  EDTA 50 -80 or -20
Montelukast 2 - 600 ng/mL Human Plasma K2  EDTA 50 -80 or -20
Morphine Morphine-3-Glucoronide Morphine-6-Glucoronide 0.1 - 100 ng/mL
5 - 3000 ng/mL
1 - 500 ng/mL		 Human Plasma K2  EDTA 100 -20
Moxifloxacin 50 - 5000 ng/mL Human Plasma Lithium Heparin 50 -20
Naloxone
Naloxone-3β-Glucoronide		 30 - 25000 pg/mL Human Plasma K2  EDTA 200 -20
Naproxen 0.25 - 100 µg/mL Human Plasma K2  EDTA 50 -20
Nateglinide 100 - 10000 ng/mL Human Plasma K2  EDTA 50 N/A
Octeotide 0.01 - 25 ng/mL Human Plasma K2  EDTA 300 -80 or -20
Olmsartan Modoxomil 1 - 1000ng/mL Human Plasma K2  EDTA 50 -80 or -20
Olodacaterol 0.4 -100 pg/mL Human Plasma K2  EDTA 500 -80 or -20
Omeprazole 1 - 500 ng/mL Human Plasma Lithium Heparin 100 -80
Oxcarbazepine & Eslicarbazepine 10 - 5000ng/mL Human Plasma K2  EDTA 25 -80 or -20
Paclitaxel 10 – 10000 ng/mL Human Plasma K2  EDTA 25 -20
Palonosertron 20 - 20000 pg/mL Monkey Urine N/A 50 N/A
Palonosertron 20 - 20000 pg/mL Monkey CSF N/A 50 N/A
Palonosertron 20 - 20000 pg/mL Monkey Plasma N/A 50 N/A
Paracetamol Low Range: 50 - 5000 ng/mL
High Range: 200 - 20000 ng/mL		 Human Plasma K2  EDTA 50 -20
Paracetamol Pseudoephedrine 50 - 5000 ng/mL
2 - 500 ng/mL		 Human Plasma Lithium Heparin 100 -20
Paroxetine 0.05 - 50 ng/mL Human Plasma K2  EDTA 50 -20
Perampanel 2 - 2000 ng/mL Human Plasma K2  EDTA 25 -80 or -20
Phenobarbital 5 - 5000 ng/mL Human Plasma K2  EDTA 50 -80 or -20
Phenytoin 20 - 10000 ng/mL Human Plasma K2  EDTA 50 -80 or -20
Posaconazole 5 - 5000 ng/mL Human Plasma Lithium Heparin 50 -20
Prasugrel (R-138727/R95913) 0.5/0.5 - 200/200
ng/mL		 Human Plasma K2  EDTA 50 -80 or -20
Pravastatin 0.5 - 250 ng/mL Human Plasma K2 EDTA 100 -20
Pregabalin 20 - 20000 ng/mL Human Plasma K2  EDTA 50 -80 or -20
8-Iso Prostaglandin F2alpha 25 - 20000 pg/mL Human Urine N/A 1000 -20
Repaglinide
3-Hydroxy Repaglinide		 0.1 - 50 ng/mL Human Plasma K2 EDTA 100 -80
Rifampicin 20 - 20000 ng/mL Human Plasma K2 EDTA 50 -80
Rifapicin 0.1 - 40 ug/mL Human Plasma K2  EDTA 25 -80 or -20
Rimonabant 1 - 600 ng/mL Human Plasma Lithium Heparin 50 -20
Ritonavir Lopinavir 4 - 3200 ng/mL
20 - 16000 ng/mL		 Human Plasma K2 EDTA 100 -20
Rosiglitazone 1 - 1000 ng/mL Human Plasma K2  EDTA 50 N/A
Rosuvastatin 0.2 - 100 ng/mL Human Plasma K3 EDTA 200 -80
Rufinamide 10-10000ng/mL Human Plasma K2  EDTA 20 -80 or -20
Scopolamine Physostigmine 0.05 - 25 ng/mL
0.1 - 50 ng/mL		 Human Plasma Lithium Heparin (with Neostigmine as stabiliser) 100 -80
Semaglutide 1 - 200ng/mL Human Plasma K2  EDTA 100 -80 or -20
Simvastatin Simvastatin Acid 0.1 - 50 ng/mL Human Plasma Lithium Heparin 100 -80
SN-38 0.2 - 20 ng/mL Human Plasma K2  EDTA 100 -80 or -20
SN-38 0.2 - 20 ng/mL Dog Plasma K2  EDTA 100 -80 or -20
D-Sorbitol 50 - 5000 ng/mL Human Blood K2  EDTA 100 -80 or -20
D-Sorbitol 50 - 5000 ng/mL Human RBC K2  EDTA 100 -80 or -20
Tacrolimus 10 - 10000 ng/mL Human Blood K2  EDTA 50 N/A
Telmisartan 2 - 1000 ng/mL Human Plasma K2  EDTA 50 N/A
Tenofovir 0.3 - 50 ng/mL Human Plasma K2  EDTA 50 -80 or -20
Teprenone 0.4 - 200 ng/mL Human Plasma K2  EDTA 400 -80 or -20
Tenofovir Alafenamide 1 - 500 ng/mL Human Plasma K2  EDTA 50 -80 or -20
Testosterone 0.05 - 30 ng/mL Human Serum N/A 100 -20
Testosterone 2 - 1000 pg/mL Human Plasma K2  EDTA 500 -80 or -20
Testosterone Dihydrotestosterone 20 - 10000 pg/mL Human Serum N/A 500 -20
Testosterone Undecanoate Dihydrotestosterone Undecanoate 1 - 500 ng/mL
1.5 - 250 ng/mL		 Human Serum N/A 200 -20
Ticlopdine 2 - 2000 ng/mL Human Serum N/A 20 -20
Tiotropium 0.25 - 50 pg/mL
0.1 - 20 pg/mL
0.1 - 50 pg/mL
0.2 - 50 pg/mL		 Human Plasma K2 EDTA 200 -20
Tiotropium/Olodaterol 0.2/0.4 - 50/100
ng/mL		 Human Plasma K2  EDTA 600 -80 or -20
Tobramycin 5 - 500 ng/mL Human Plasma Lithium Heparin 50 -80
Tolvaptan 1 - 1000 ng/mL Human Plasma Sodium Heparin 50 -20
Topiramate 10 - 10000 ng/mL Human Plasma K2  EDTA 25 -80 or -20
Umeclidinium/Vilanterol 10 - 2000 pg/mL Human Plasma K2  EDTA 500 N/A
Valproic Acid 2 - 100 ug/mL Human Plasma K2  EDTA 25 -80 or -20
VK1 (trans, Cis)-low range 0.1/0.1 - 50/50 ng/mL Human Plasma K2  EDTA 400 -80 or -20
VK1 (trans, Cis)-high range 10/10 - 3000/3000
ng/mL		 Human Plasma K2  EDTA 50 -80 or -20
Warfarin (R and S) 10 - 2000 ng/mL Human Plasma K2 EDTA 1000 -20
Zonisamide 100 - 50000 ng/mL Human Plasma K2  EDTA 25 -80 or -20