Bioanalytical LC-MS/MS Method List
With 20+ years of working to GLP and GCP, we have developed an extensive number of LC-MS/MS bioanalytical methods in a wide range of species and matrices. These have been established/validated in line with the latest EMA/FDA guidance and applied in the quantitation of therapeutic drugs, metabolites and biomarkers.
Assay Experience
The following list details our bioanalytical LC-MS/MS method experience.
Some of these methods may require re-establishment or validation prior to use.
We have also developed and validated many more proprietary methods across a broad range of species and matrices.
If an analyte of interest is not listed, please get in touch and we can discuss setting up a method to support your project requirements.
| Analyte name | Calibration curve range (LLOQ-ULOOQ) |
Species | Matrix | Anticoagulant | Sample volume (μL) |
Storage temperature | |||||||||
| Aldosterone | 5 - 500 pg/mL | Human | Plasma | K2 EDTA | 300 | -80 | |||||||||
| Aldosterone | 0.1 - 50 ng/mL | Human | Urine | N/A | 100 | -80 | |||||||||
| Androstenedione | 0.1 - 100 ng/mL | Human | Plasma | K2 EDTA | 200 | -20 | |||||||||
| Angiotensin I (Plasma Renin Activity) |
0.2 - 200 ng/mL | Human | Plasma | K2 EDTA | 100 | TBC | |||||||||
| Aripiprazole Olanzapine Quetiapine Risperidone Paliperidone |
5 - 500 ng/mL 0.2 - 100 ng/mL 5 -500 ng/mL 0.1 - 100 ng/mL 0.2 - 100 ng/mL |
Human | Plasma | K2 EDTA | 100 | -20 | |||||||||
| Atorvastatin 2-Hydroxy Atorvastatin 4-Hydroxy Atorvastatin |
20 - 20000 pg/mL 20 - 20000 pg/mL 10 - 1000 pg/mL |
Human | Plasma | K2 EDTA | 100 | -20 | |||||||||
| Budesonide | 10 - 10000 pg/mL | Human | Plasma | NaF / KOx | 250 | -20 | |||||||||
| Buprenorphine Norbuprenorphine | 2 -1000 pg/mL 5 - 1000 pg/mL |
Human | Plasma | K2 EDTA | 400 | -20 | |||||||||
| C4 (7 -hydroxy-4-cholensten-3-one) |
2 -500 ng/mL | Human | Plasma | Lithium Heparin | 100 | -20 | |||||||||
| Ciprofloxacin | 100 - 4500 ng/mL | Human | Plasma | Lithium Heparin | 50 | -20 | |||||||||
| Clopidogrel Clopidogrel acid |
10 - 5000 pg/mL 30 - 4000 ng/mL |
Human | Plasma | Lithium Heparin | 100 | -80 | |||||||||
| Clozapine | 5 -1000 ng/mL | Human | Plasma | K2 EDTA | 50 | -20 | |||||||||
| Cortisol | 1 - 500 ng/mL | Human | Plasma | K2 EDTA | 200 | -20 | |||||||||
| Cortisol | 0.2 - 20 ng/mL | Human | Saliva | N/A | 200 | -20 | |||||||||
| Cortisol | 0.6 - 300 ng/mL | Human | Urine | N/A | 200 | -20 | |||||||||
| Cortisol 11-Deoxycortisol Corticosterone 11-Deoxycorticosterone |
1 - 250 ng/mL 20 - 2000 pg/mL 50 - 25000 pg/mL 20 - 1000 pg/mL |
Human | Plasma | K2 EDTA | 200 | -80 | |||||||||
| Cyclosporin A Tacrolimus Sirolimus |
1 - 500 ng/mL 0.1 - 50 ng/mL 0.1 - 50 ng/mL |
Human | Whole Blood | K2 EDTA | 50 | -80 | |||||||||
| Desmosine | 0.25 - 100 nM (~0.13 - ~53 ng/mL) |
Human | Urine | N/A | 300 | -20 | |||||||||
| Desmosine Isodesmosine |
30 - 2000 pM (~16 - ~1050 pg/mL) |
Human | Serum | N/A | 500 | -20 | |||||||||
| Diazepam Desmethyldiazepam |
0.5 - 500 ng/mL 0.1 - 100 ng/mL |
Human | Plasma | K2 EDTA | 50 | -20 | |||||||||
| Digoxin | 10 - 5000 pg/mL | Human | Plasma | Lithium Heparin | 100 | -20 | |||||||||
| Duloxetine | 1 - 500 ng/mL | Human | Plasma | Lithium Heparin | 100 | -20 | |||||||||
| Enoxacin | 5-5000ng/mL | Human | Plasma | K2 EDTA | 50 | -20 | |||||||||
| Estradiol | 5 - 1000 pg/mL | Human | Serum | N/A | 500 | -20 | |||||||||
| Ethinylestradiol Levonorgestrel |
10 - 2000 pg/mL 0.1 - 20 ng/mL |
Human | Plasma | Sodium Heparin | 300 | -20 | |||||||||
| Fluconazole | 50 - 4000 ng/mL | Human | Plasma | Lithium Heparin | 50 | -20 | |||||||||
| Fluconazole | 50 - 50000 ng/mL | Human | Plasma | K2 EDTA | 20 | -20 | |||||||||
| Fluticasone Propionate | 1 - 500 pg/mL | Human | Plasma | NaF/KOx | 500 | -20 | |||||||||
| Fluticasone Propionate (High Range) Salmeterol |
5 - 1000 pg/mL 1 - 250 pg/mL |
Human | Plasma | NaF/KOx | 500 | -20 | |||||||||
| Fluticasone Propionate (Low Range) Salmeterol |
1 - 250 pg/mL | Human | Plasma | NaF/KOx | 500 | -20 | |||||||||
| Fluvastatin | 1 - 1000 ng/mL | Human | Plasma | K2 EDTA | 50 | -20 | |||||||||
| Fluvoxamine | 5-1200 ng/ml | Human | Plasma | K2 EDTA | 50 | -20 | |||||||||
| Formoterol | 0.250 - 125 pg/mL | Human | Plasma | NaF/KOx | 500 | -20 | |||||||||
| Furosemide | 10 - 5000 ng/mL | Human | Plasma | Lithium Heparin | 100 | -20 | |||||||||
| Gemfibozil Gemfibrozil 1-O-β-glucuronide |
50 - 25000 ng/mL | Human | Plasma | K2 EDTA | 20 | -20 | |||||||||
| Glucagon | 15 - 250 pg/mL | Human | Plasma | K3 EDTA | 200 | -80 | |||||||||
| Hydromorphone Naloxone Hydromorphone-3β-Glucuronide Naloxone-3β-Glucuronide |
10 - 2500 pg/mL 100 - 2500 pg/mL 100 - 25000 pg/mL Low range: 100 - 25000 pg/mL High range: 1000 - 250000 pg/mL |
Human | Plasma | K2 EDTA | 200 | -20 | |||||||||
| 4β-Hydroxycholesterol | 1 - 500 ng/mL | Human | Plasma | K2 EDTA | 50 | -80 | |||||||||
| Ibuprofen | 1 - 100 µg/mL | Human | Plasma | Lithium Heparin | 50 | -20 | |||||||||
| Ibuprofen (R and S) | 0.1 - 50 µg/mL | Human | Plasma | Lithium Heparin | 50 | -80 | |||||||||
| Itraconazole Hydroxyitraconazole |
5 -2000 ng/mL | Human | Plasma | K2 EDTA | 50 | -20 | |||||||||
| Levodopa Carbidopa 3-OMD |
50 - 7500 ng/mL 10 - 1500 ng/mL 200 - 30000 ng/mL |
Human | Plasma | K2 EDTA | 50 | -80 | |||||||||
| Levofloxacin | 20 - 20000 ng/mL | Human | Plasma | Sodium Heparin | 50 | -80 | |||||||||
| LTE4 (Leukotriene E4) | 8 - 500 pg/mL | Human | Urine | N/A | 1000 | -80 | |||||||||
| Midazolam 1-OH Midazolam 4-OH Midazolam |
0.1 - 100 ng/mL | Human | Plasma | K2 EDTA | 100 | -20 | |||||||||
| Morphine Morphine-3-Glucoronide Morphine-6-Glucoronide |
0.1 - 100 ng/mL 5 - 3000 ng/mL 1 - 500 ng/mL |
Human | Plasma | K2 EDTA | 100 | -20 | |||||||||
| Moxifloxacin | 50 - 5000 ng/mL | Human | Plasma | Lithium Heparin | 50 | -20 | |||||||||
| Naloxone Naloxone-3β-Glucoronide |
30 - 25000 pg/mL | Human | Plasma | K2 EDTA | 200 | -20 | |||||||||
| Naproxen | 0.25 - 100 µg/mL | Human | Plasma | K2 EDTA | 50 | -20 | |||||||||
| Omeprazole | 1 - 500 ng/mL | Human | Plasma | Lithium Heparin | 100 | -80 | |||||||||
| Paclitaxel | 10 – 10000 ng/mL | Human | Plasma | K2 EDTA | 25 | -20 | |||||||||
| Paracetamol | Low Range: 50 - 5000 ng/mL High Range: 200 - 20000 ng/mL |
Human | Plasma | K2 EDTA | 50 | -20 | |||||||||
| Paracetamol Pseudoephedrine |
50 - 5000 ng/mL 2 - 500 ng/mL |
Human | Plasma | Lithium Heparin | 100 | -20 | |||||||||
| Paroxetine | 0.05 - 50 ng/mL | Human | Plasma | K2 EDTA | 50 | -20 | |||||||||
| Posaconazole | 5 - 5000 ng/mL | Human | Plasma | Lithium Heparin | 50 | -20 | |||||||||
| Pravastatin | 0.5 - 250 ng/mL | Human | Plasma | K2 EDTA | 100 | -20 | |||||||||
| 8-Iso Prostaglandin F2alpha | 25 - 20000 pg/mL | Human | Urine | N/A | 1000 | -20 | |||||||||
| Repaglinide 3-Hydroxy Repaglinide |
0.1 - 50 ng/mL | Human | Plasma | K2 EDTA | 100 | -80 | |||||||||
| Rifampicin | 20 - 20000 ng/mL | Human | Plasma | K2 EDTA | 50 | -80 | |||||||||
| Rimonabant | 1 - 600 ng/mL | Human | Plasma | Lithium Heparin | 50 | -20 | |||||||||
| Ritonavir Lopinavir |
4 - 3200 ng/mL 20 - 16000 ng/mL |
Human | Plasma | K2 EDTA | 100 | -20 | |||||||||
| Rosuvastatin | 0.2 - 100 ng/mL | Human | Plasma | K3 EDTA | 200 | -80 | |||||||||
| Scopolamine Physostigmine |
0.05 - 25 ng/mL 0.1 - 50 ng/mL |
Human | Plasma | Lithium Heparin (with Neostigmine as stabiliser) | 100 | -80 | |||||||||
| Simvastatin Simvastatin Acid |
0.1 - 50 ng/mL | Human | Plasma | Lithium Heparin | 100 | -80 | |||||||||
| Testosterone | 0.05 - 30 ng/mL | Human | Serum | N/A | 100 | -20 | |||||||||
| Testosterone Dihydrotestosterone | 20 - 10000 pg/mL | Human | Serum | N/A | 500 | -20 | |||||||||
| Testosterone Undecanoate Dihydrotestosterone Undecanoate | 1 - 500 ng/mL 1.5 - 250 ng/mL |
Human | Serum | N/A | 200 | -20 | |||||||||
| Ticlopidine | 2 - 2000 ng/ml | Human | Plasma | K2 EDTA | 20 | -20 | |||||||||
| Tiotropium | 0.25 - 50 pg/mL | Human | Plasma | K2 EDTA | 200 | -20 | |||||||||
| Tobramycin | 5 - 500 ng/mL | Human | Plasma | Lithium Heparin | 50 | -80 | |||||||||
| Tolvaptan | 1 - 1000 ng/mL | Human | Plasma | Sodium Heparin | 50 | -20 | |||||||||
| Warfarin (R and S) | 10 - 2000 ng/mL | Human | Plasma | K2 EDTA | 1000 | -20 | |||||||||
